Skip to main content
. 2016 Sep 9;11(9):e0161883. doi: 10.1371/journal.pone.0161883

Fig 1. Experimental design.

Fig 1

(A) Experiment I: animals infected with Theiler’s murine encephalomyelitis virus (TMEV) received IL-10 receptor blocking antibodies (IL-10R Ab) once weekly at day 0, 7, 14 and 21 of the experiment (grey box). Necropsy (†) was performed in groups of five animals at 7, 14, 22 and 42 days post infection (dpi). For negative controls a second group received IgG1-specific isotype control instead of IL-10R Ab (*) and a third group was mock-infected only (#) (B) Experiment II: TMEV-infected animals received IL-10R Ab once weekly at 35 and 42 dpi (grey box). Necropsy (†) was performed in groups of five animals at 42 and 49 dpi. For negative controls, a second group received IgG1-specific isotype control instead of IL-10R Ab (*) and a third group was mock-infected only (#) (C) Experiment III: animals received IL-10R Ab once weekly at day 0, 7 and 14 (grey box). Necropsy (†) was performed in groups of five animals at 7, 14 and 21 days after the first Ab application. For negative control a second group received IgG1-specific isotype control instead of IL-10R Ab (*).