Table 1. Summary of antibodies used for immunohistochemistry.
| Ab | Primary Ab | Pre-treatment and dilution | Specifity |
|---|---|---|---|
| CD3 A0452 | DakoCytomation polyclonal Ab rabbit | Citrate buffer / microwave 1:1000 | T cells |
| CD45R/B220 553085 clone RA3-6B2 | BD Biosciences monoclonal Ab rat | Citrate buffer / microwave 1:1000 | B cells |
| Foxp3 14-5773 clone FJK-16s | eBioscience monoclonal Ab rat | Citrate buffer / microwave 1:50 | Treg |
| CD107b MCA2293B clone M3/84 | AbD Serotec monoclonal Ab rat | Citrate buffer / microwave 1:200 | Activated macrophages/microglia |
| Iba-1 019-19741 | Wako Pure Chemical Industries polyclonal Ab rabbit | Citrate buffer / microwave 1:500 | Macrophages |
| Arginase-1 sc-18351 | Santa Cruz Biotechnology polyclonal Ab goat | Citrate buffer / microwave 1:50 | Activated M2-type macrophages / microglia |
| Np-NF 801703 clone SMI 311 | BioLegend monoclonal Ab goat | Citrate buffer / microwave 1:8000 | Axonal damage |
| MBP AB980 | Merck Millipore polyclonal Ab rabbit | No pretreatment 1:2000 | MBP |
| TMEV | Kummerfeld et al. 2009 polyclonal Ab rabbit | No pretreatment 1:2000 | TMEV BeAn |
Ab = antibody, Foxp3 = forkhead box p3; Treg = regulatory T cells; Iba-1 = ionized calcium binding adaptor molecule 1; np-NF = non phosphorylated neurofilament; MBP = myelin basic protein; TMEV = Theiler’s murine encephalomyelitis virus.