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. 2016 Sep 9;11(9):e0162568. doi: 10.1371/journal.pone.0162568

Fig 3. Inhibition of mitochondrial respiration by compound 7.

Fig 3

(A) Effect of compound 7 on mitochondrial respiration. OCR was measured using XF24 extracellular flux analyzer by adding oligomycin (1 μM), FCCP (0.5 μM), and rotenone (1 μM)/antimycin A (1 μM) to compound 7-treated HCT116 cells. (B) Effect of compound 7 on intracellular oxygen tension. HCT116 cells were treated with compound 7 for 6 h under hypoxia condition. The intracellular oxygen tension was detected by hypoxia-sensitive probe MAR (0.5 μM). The scale bar indicates 200 μm. The fluorescence intensity of MAR probe was shown by vertical bars. Statistical significance: **P < 0.01, compared with hypoxia control.