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. 2016 Sep 9;11(9):e0162568. doi: 10.1371/journal.pone.0162568

Fig 4. Inhibition of hypoxia-induced HIF-1α activation and tumor growth by compound 7.

Fig 4

(A) Effect of compound 7 on HIF-1α activation. The hypoxia-induced transcriptional activation of HIF were determined by a cell-based HRE reporter gene assay. HCT116 cells stably expressing HRE-luciferase reporter gene were incubated for 12 h with or without drugs under normoxic or hypoxic conditions. After removing the supernatant, luciferase activity was measured. Statistical significance: **P < 0.01, compared with untreated control. (B) Effects of compound 7 on hypoxia-induced HIF-1α accumulation. The protein levels in compound 7-treated cells were detected by immunoblot analysis in HCT116 cells. (C) Effects of compound 7 on expression of mRNA level of HIF-1α target genes. The mRNA levels were detected by RT-PCR analysis. (D) Inhibitory effects of compound 7 on HIF-1α accumulation in various cancer cells.