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. 2016 Sep 9;11(9):e0162675. doi: 10.1371/journal.pone.0162675

Fig 1. Survival functions and lifespan of flies.

Fig 1

(A) Survival functions of CS. (B) Survival functions of w1118. (C) Average lifespan of flies. (D) Median lethal time of flies. (E) Maximum lifespan of flies. Kaplan-Meier survival analysis was conducted, and log-rank (Mantel-Cox) test was performed to analyze significant differences among groups. Time durations from the start of stress exposure to the last recorded activity were considered to analyze their lifespan. Ages of 50% and 90% mortality were used as the evaluation indexes of median lethal time and maximum lifespan, respectively. Data are presented as mean ± SEM. Asterisk (*) indicates significant difference between the male and female flies of both lines exposed to 35°C and 35°C + ELF, respectively (*P < 0.05, **P < 0.01). Flies of three replicates were used for analysis. The numbers of CS and w1118 flies were as follows: 25°C group (♀: n = 96, ♂: n = 94); 35°C group (♀: n = 128, ♂: n = 128); and 35°C + ELF group (♀: n = 124, ♂: n = 124).