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. 2016 Sep 9;11(9):e0162886. doi: 10.1371/journal.pone.0162886

Fig 2. Size-exclusion chromatography of TreA.

Fig 2

TreA was subjected to Size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL column (GE Healthcare Life Sciences) at flow rate 0.5 mL/min in 25 mM Tris-HCl pH 8.0, 50 mM NaCl, and monitored by absorbance at 280 nm. (A) Chromatogram indicating TreA major elution peak around 15 mL. (B) Partition coefficients (Kav) of TreA and proteins used in the SEC calibration curve. TreA elution was modeled as a monomer or dimer and the results indicate that TreA must be a monomer. Pearson's linear correlation coefficient was 0.996 for the calibration curve.