Table 3. Average percentages of SpeB^A- variants selected from invasive and pharyngeal emm1 SpeB^A+ isolates and P values in statistical analysis.

Strain number	Infection	Avg. SpeBA- variants (%)a	Group	Significant P valueb
1921	Invasive	96.5		iG1 vs iG2: <0.0001; iG1 vs iG4: ≤0.0372; iG1 vs pG2: <0.0001; iG1 vs pG4: ≤0.0216; iG2 vs pG1: ≤0.0003; iG3 vs iG2: <0.05
1964	Invasive	85.8	iG1
1980	Invasive	91.3
1841	Invasive	5.0
1969	Invasive	3.5	iG2
1993	Invasive	7.9
1856	Invasive	54.6
1866	Invasive	56.2
1888	Invasive	61.3
1896	Invasive	55.0
1901	Invasive	41.2
1927	Invasive	57.9	iG3
1928	Invasive	47.9
1931	Invasive	62.1
1962	Invasive	52.5
1970	Invasive	36.1
1979	Invasive	45.0
1982	Invasive	52.5
1922	Invasive	12.5
1932	Invasive	26.7
1925	Invasive	18.8	iG4
1940	Invasive	30.0
1955	Invasive	21.3
1972	Invasive	30.8
2775	Pharyngeal	81.2	pG1	pG1 vs pG2: <0.0001; pG1 vs pG3: ≤0.0119; pG1 vs pG4: ≤0.0059; pG2 vs pG3: ≤0.0405
2743	Pharyngeal	1.7	pG2
2749	Pharyngeal	0
2756	Pharyngeal	46.0	pG3
2757	Pharyngeal	39.0
2745	Pharyngeal	29.4
2738	Pharyngeal	13.3	pG4
2779	Pharyngeal	25.0

^aThe average SpeB^A-% values were calculated from the data in Figs 1 and 2.

^bP values were from the analysis of the SpeBA-% data using one way ANOVA Tukey’s multiple comparison test