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. 2016 Sep 9;11(9):e0162467. doi: 10.1371/journal.pone.0162467

Fig 6. Comparison of different PCR methods on patient-derived muscle cell cultures.

Fig 6

Quantification of exon 51 skip levels in patient-derived muscle cell cultures (Δ48–50), transfected with an exon 51 skipping AON (400nM) by (A) ddPCR analysis, (B) qPCR analysis and (C) conventional primary PCR (20/30/40 cycles) and nested PCR (44 cycles). (D) Low background levels of exon 51 skipping were detected by ddPCR and qPCR in the non-treated (NT) samples. N/a indicates that no exon skip percentage could be calculated. Percentages shown are mean (n = 3) with SEM.