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The cells were treated with 10ng/ml of TGF-β1 for different indicated periods, from 15 minutes to 24 hours. (a) PP2Ac protein expression was measured by Western blot analysis. (b) PP2Ac activity was detected by using the Serine/Threonine Phosphatase Assay System in TGF-β1-treated HUVECs (n≥3). *P<0.05 compared to control group. Bars represented means ± SEM.
