Fig 6. Ipilimumab (Anti-CTLA-4) and Nivolumab (Anti-PD-1) Antibodies Potentiate IL-2 Secretion in SEB-Stimulated Human PBMC.
Frozen PBMC from 10 normal healthy donors were seeded at 1×105 cells/well and stimulated with SEB titrated 30-fold from 2.5 μg/mL. Ipilimumab, nivolumab, or isotype control antibody (1D12g4) were present at 10 μg/mL each. IL-2 secretion was measured by ELISA (in triplicate) on day 3. A. Dose titration data from one donor. B. Pooled data from 10 donors are plotted as fold-change over IL-2 values for SEB at 85 ng/mL. Bar = two-tailed T test p = 0.0088.