Fig 5.

Regression analysis of UCK1/UCK2 expression and enzyme activity for [2-¹⁴C]-uridine and [³H]-RX-3117 in the cell line panel (A and B) and xenograft cells (C and D). In both panels UCK2 mRNA expression correlated significantly (p<0.001) with UCK enzyme activity measured with both [2-¹⁴C]-uridine (r = 0.828 for the cell lines and r = 0.878 for the xenograft cells) and [³H]-RX-3117 (r = 0.803 for the cell lines and r = 0.915 for the xenograft cells). UCK1 mRNA expression did not correlate with enzyme activity with either substrate (r = -0.721 and -0.585, respectively for the cell lines and r = -0.119 and r = -0.056 respectively for the xenograft cells). The cell lines in panel A and B shown in increasing UCK activity are: U937, A549, CCRF-CEM, SW1573, AG6000 and A2780. In panel C and D the sequence is LS174T, BT474, RT112, Raji B, SW780, PANC-1 and C-33A. The r-coefficient is given in the legend along for each gene. Significant correlations are marked with an asterisk (*).