Fig 1. Isolation of exosomes.

(A) Representative transmission electron microscopy images of exosome samples. Exosomes were isolated by ultracentrifugation and ExoQuick, and analyzed by electron microscopy. Scale bar = 100 nm. (B) PCR and western blotting analysis of isolated exosomes. Virus particles of KSHV and EBV and cell lysate of Bjab cells were also examined. KSHV KS330₂₃₃, EBV W and beta-globin genes were amplified with PCR (upper panels). A faint band of KSHV is found in the exosome sample from BCBL-1 in PCR. KSHV ORF45, Lyn, CD63, HSP70, and Lyn were detected in isolated exosome samples with western blotting analysis (lower panels). A faint band of KSHV ORF45 is observed in the exosome sample from BCBL-1.