Fig 3. PPARα agonist increased the expression of catalase and ameliorated UV-induced ROS generation in human dermal fibroblasts.

Cultured fibroblasts were serum-starved for 24 h, and treated with 1–100 μM Wy14643 for 72 h. (a) Total soluble proteins were isolated from the cells, and levels of catalase protein were measured by western blot analysis and normalized to β-actin. (b) Catalase mRNA expression was determined by qPCR. Data are presented as means±SEM (n = 3), *p<0.05 vs. control. (c) Catalase activity was measured by a spectrophotometric method. Catalase enzyme unit is equivalent to 1 μmol of substrate disappearance or product formation per min. Data are presented as mean±SEM (n = 3). (d) Human dermal fibroblasts were treated with Wy14643 for 72 h, and then irradiated with UV. Subsequently, cells were treated with 2,7-dichlororofluorescein diacetate (DCFDA) and assayed using a fluorescence reader. Data are presented as means±SEM (n = 6), *p<0.05, ***p<0.001 vs. control, #p<0.05 vs. UV-treated cells.