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. 2016 Sep 9;11(9):e0162584. doi: 10.1371/journal.pone.0162584

Fig 3. Effects of miR-9 knockdown on biological responses of OCCC cells.

Fig 3

(A) JHOC-9 and OVISE cells transfected with either a miR-9 inhibitor or anti-miR-NC were subjected to an invasion assay. All experiments were repeated at least three times, and values are presented as means ± SDs. **P < 0.001. Representative images are shown. (B) E-cadherin, Vimentin, Fibronectin, and MMP-9 protein expressions in JHOC-9 and OVISE cells that were transfected with either miR-9 inhibitor or anti-miR-NC were analyzed by Western blotting. β-Actin expression was used as a loading control. (C) A luciferase reporter assay using the pMIR-REPORT™ System was conducted to confirm direct binding of miR-9 to CDH1 (E-cadherin) 3′-UTR in JHOC-9 and OVISE cells. All experiments were repeated at least three times, and values are presented as means ± SDs. *P < 0.05, **P < 0.001.