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. 2016 Sep 9;12(9):e1006274. doi: 10.1371/journal.pgen.1006274

Fig 1. Subunit switching in the BAF complex during differentiation of oNSCs to ORNs.

Fig 1

(A) Expression of BAF subunits in oNSCs and ORNs. Cell lysates were prepared from cultured oNSCs and ORNs, and blotted with BAF subunit-specific antibodies. (B) Co-immunoprecipitation of nuclear extracts from cultured oNSCs and ORNs using anti-Brg1/Brm antibodies and Western blot analyses with antibodies against BAF subunits revealed that BAF subunits integrate into Brg1/Brm-based BAF complexes. (C, D) Double-immunostaining of coronal sections of the OE from E12.5 mice revealed non-overlapping expression of oNSC-specific BAF45a/BAF53a subunits and ORN-specific BAF45b/BAF53b subunits in the developing OE. (E) A switch in subunits of BAF complexes during differentiation from oNSCs to ORNs. Abbreviations: oNSC, olfactory neural stem cell, ORN, olfactory receptor neuron; D/V, dorsal/ventral. Scale bars = 50 μm (C, D).