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. 2016 Sep 9;11(9):e0162504. doi: 10.1371/journal.pone.0162504

Fig 1. Expression of Mogat1 in various mouse tissues and cell lines.

Fig 1

A. Mogat1 expression in several mouse tissues. Mogat1 is expressed in brown adipose tissue, epididymal fat, kidney and stomach. B. Mogat1 expression was measured by RT-qPCR in the brown adipose tissue (BAT), epidydymal fat, kidney and stomach obtained from WT mice. The data were normalized to cyclophilin and then expressed as a fold change increase in Mogat1 compared to kidney. Stomach was found to be the highest expressing tissue. C. MOGAT1 is not expressed in human embroyonic kidney (HEK-293) cells but is expressed in human proximal kidney tubule (HK-2) cells and normal rat kidney cells (NRK). D. Other human cell lines screened for MOGAT1 expression: Caco-2, HT-29, Huh-7 and MCF-7. E. Expression of Mogat1 in rodent cell lines: mouse fibroblasts, 3T3-L1; Chinese hamster ovary (CHO) cells; Rat hepatic tumor cells, HTC. Mogat1 was expressed only in HTC cells. Shown is the Mogat1 product as analyzed on 1.5% agarose gels and expression of the housekeeping gene glyceradehyde phosphate dehydrogenase (Gapdh). (+) = cDNA amplification in the presences of reverse transcriptase; (-) = cDNA amplification in the absence of reverse transcriptase.