Skip to main content
. 2016 Sep 9;11(9):e0162504. doi: 10.1371/journal.pone.0162504

Fig 2. Schematic of human and mouse MOGAT1 gene and product size.

Fig 2

A. The human MOGAT1 gene consists of 6 exons, marked as boxes. Shown below is the predicted MGAT protein. B. Amplification of human MOGAT1 transcript from HK-2 cells produce a product of ~700 bp and upon sequencing confirmed the deletion of exon 3. C. Northern blot analysis of total RNA from HK-2 cells probed with human MOGAT1 showing the expected size of mRNA. Northern analysis confirmed the presence of a single MOGAT1 transcript in HK-2 cells. D. Amino acid alignment of the predicted MOGAT1 protein with truncated (exon 3 deleted) MOGAT1 protein in human kidney confirmed by sanger sequencing. This will result in a frame shift producing a truncated protein of p.Leu91Glyfs*13 and deletion of the putative catalytic site HPHG, marked with an asterisk. E. Schematic of mouse Mogat1 gene showing exons marked as boxes. Shown below is the predicted MGAT protein. Mouse Mogat1 has an additional untranslated exon shown as 1a. F. Amplification of mouse Mogat1 mRNA of expected size and upon sequencing confirmed the predicted sequence.