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. 2016 Sep 9;11(9):e0162504. doi: 10.1371/journal.pone.0162504

Fig 9. Chromatin immunoprecipation (ChIP) assay for binding of peroxisome proliferator response element (PPRE) located in MOGAT1 promoter.

Fig 9

A. Schematic of human MOGAT1 promoter showing the two PPRE sites located at -349 (site 1) and -1998 (site 2) bp in relation to ATG. Circles represent PPRE sites, the orange box is exon 1 and the arrow head represents the transcriptional start site (TSS). B. Shown are the locations and sequences of the PPRE sites found in the human promoter. C. Transient transfection of human MOGAT1 promoter construct in HK-2 cells. Bars represent the fold changes in relative luciferase activity shown as mean±SEM (n = 3) performed in duplicate. D. ChIP assay in cell lysate from HK-2 cells probed with PPARα antibody and the amplified product analyzed on 1.5% agrose gel. Among the two PPRE sites only site 1 located at -349 was occupied by PPARα. Shown is the representative image from replicate experiments (n = 2).