Fig. 4.

Distribution of genes involved in methylamine and methanol metabolism among Methylobacterium species and the ability of Methylobacterium strains to use methylamine and methanol as sole carbon sources. (A) Percentage of strains in each group that have genes responsible for each step; pathway maps are shown next to the graphs. Abbreviations for enzymes: Fae, formaldehyde-activating enzyme; Fch, methenyl-H₄F cyclohydrolase; FDH, formate dehydrogenase; Fhc, formyltransferase/hydrolase complex; FtfL, formate-tetrahydrofolate ligase; Mch, methenyl-dH₄MPT cyclohydrolase; MDH, methanol dehydrogenase; MtdA and MtdB, methylene-tetrahydromethanopterin dehydrogenase. Abbreviations for compounds: CH₂=dH₄MPT, methylene-dH₄MPT; CH≡dH₄MPT, methenyl-dephospho H₄MPT; CHOdH₄MPT, formyl dH₄MPT; MFR, methanofuran; CHOMFR, formyl-MFR; H₄F, tetrahydrofolate; CHOH₄F, formyl-H₄F; CH≡H₄F, methenyl H₄F; dH₄MPT, dephosphotetrahydromethanopterin; GMA, γ-glutamylmethylamide; NMG, N-methylglutamate; α-KG, α-ketoglutarate. (B) Growth test without a carbon source or with methylamine or methanol as the sole carbon source for 7 d. 5, Methylobacterium sp. AMS5 (Group I); 1, M. extorquens AM1 (Group I); C, M. oryzae CBMB20 (Group III); J, M. radiotolerans JCM2831 (Group III).