Fig. 3.

Comparison of nitrogenase-like gene cassettes found in Syntrophothermus lipocalidus strain TGB-C1, Thermacetogenium phaeum strain PB, Clostridium butyricum strain CWBI1009, and Syntrophobacter fumaroxidans strain MPOB. Strain TGB-C1 nitrogenase-associated cassette encodes nitrogen fixation proteins (NifBDEHIK), glutamine amidotransferase class-I (gla), a molybdate transporter (ModABC), and ammonia transporter (amt) with a nitrogen regulatory protein P-II family (glnB). Other strains harbor NifV (strains PB and CWBI1009), peptidyl-prolyl cis-trans isomerase C (ppiC) (strain PB), ferredoxin (strain MPOB), and hypothetical protein (hyp) (strains PB and CWBI1009) within the cassette. Abbreviated locus tags are shown (e.g., ‘Slip_2130’ as ‘2130’ in the row of strain TGB-C1). Figures in gene boxes indicate amino acid sequence identity to the corresponding gene of strain TGB-C1.