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. 2016 Jul 23;31(3):299–306. doi: 10.1264/jsme2.ME15209

Table 1.

Summary of isolation steps from two samples collected in February and July 2012

Media February 2012 July 2012


CFU No. of colonies CFU No. of colonies




Total Colored Colored* BChl** Total Colored Colored* BChl**
PEA n.t. n.t. n.t. n.t. 7.6×104 6.0×104 10 2
1/10PEA 1.5×105 1.2×105 8 8 8.4×104 3.4×104 12 6
1/10PEG 1.0×105 2.0×104 2 1 2.0×104 2.9×103 2 0
1/100PEA 3.1×104 6.7×103 2 2 6.2×104 2.1×104 5 4
1/100PEG 2.0×104 6.7×103 5 2 4.0×104 1.5×104 8 2
NA n.t. n.t. n.t. n.t. 3.3×104 2.9×104 1 0
1/6NA n.t. n.t. n.t. n.t. 4.4×104 2.7×104 7 0
1/60NA n.t. n.t. n.t. n.t. 6.1×104 2.4×104 6 1

Media; PEA, PEG, PE medium supplemented with agar or gellan gum, respectively; 1/10, 1/100, 10, or 100 times diluted version of the media; NA, nutrient agar; 1/6NA, 1/60NA, 6, or 60 times diluted NA supplemented with agar up to 1.5% CFU; Total, CFU (cm−2 biofilm) calculated by counting the total number of colonies; Colored, CFU (cm−2 biofilm) calculated by counting the number of red-, pink-, yellow-, orange- or brown-colored colonies.

n.t., Not tested

*

Number of colored colonies tested to have bacteriochlorophyll

**

Number of bacteriochrolophyll-containing colonies in colored colonies tested