Table 1. Table of computationally determined peptides for solution assembly.

Colored rectangles contain eight candidate sequences that were experimentally characterized. Sequences were theoretically designed to produce tetrahelical bundles. BNDL_1 was designed in the absence of any lattice assembly and is expected to remain soluble (brown). The P222 (orange), P422 (green), and P622 (blue) sequences were designed in the presence of lattices of corresponding symmetry. The remaining P222_9 and P422_1 sequences contain covalently modified termini. P222_4 is the only sequence candidate that did not behave as predicted and could not be assembled into a nanostructure in the solution conditions used for the other peptides. The heptad repeat positions (abcdefg) of all peptides are shown in the table heading. The designed, hydrophobic interior residues of the motif shown are highlighted in gray. MW, molecular weight.