Fig. 2.

In situ hybridization studies of POSTN mRNA expression in normal palmar fascia and DD cord tissues. In situ hybridization studies performed using S³⁵ labeled sense and antisense transcripts derived from a 391-bp fragment from the 5′ end of a POSTN cDNA. Paraffin-embedded DD cord tissues (A, B, E and F) and palmar fascia tissue adjacent to the cord (C and D) were exposed to antisense POSTN mRNA probe (A–D) or sense POSTN mRNA probe control (E and F). Bright field images of hematoxylin and eosin stained tissues are shown in A, C and E while the same fields under dark field are shown in B, D and F. Silver grains deposited due to exposure to the S³⁵ labeled transcripts are evident as uniform white specks under dark field. As shown, densely cellular DD cord tissue (B) exhibits intense antisense POSTN mRNA probe signal relative to low-level signal in adjacent palmar fascia (D) or densely cellular DD cord tissue exposed to sense POSTN mRNA probe (F). All images are 400×.