Fig 1. Conversion of 12-oxophytodienoic acid (OPDA) in leaves of Arabidopsis thaliana ecotype WS (wild type, A) and opr3 (B).
A, Leaves of 5-week-old WS plants grown under short-day conditions were floated on water (control, black bars) or on 50 μM OPDA for the time periods indicated. Levels of OPDA, jasmonic acid (JA) and JA-Ile are presented. B, Leaves of 5-week-old opr3 plants grown under short-day conditions were floated on solutions containing different concentrations of OPDA for 30 min. Levels of OPDA (red bars), JA (blue bars) and JA-Ile (green bars) are presented. Contents of OPDA, JA and JA-Ile were determined by LC-MS according to [30]. Each value is represented by the mean of three independent biological replicates ± SD. Treatments and controls were pairwise compared by the Student’s t-test, *p≤0.05, **p≤0.01, ***p≤0.001.