Figure 2.

Lypd6 binds multiple nAChR subunits in the human brain. (a) Cortical homogenates from human temporal cortex (input) were affinity purified using magnetic beads covalently coupled with recombinant human Lypd6 (Lypd6) or non‐coupled beads (ctrl). Also shown is the remaining homogenate after affinity purification (output). Samples were submitted to gel electrophoresis and western blotting followed by detection of nAChR subunits and the GluR2 AMPA receptor subunit. (b) Representative images of western blots of Lypd6 affinity purification of human temporal cortex homogenates pre‐incubated with 100 nM α‐BTX or vehicle [phosphate‐buffered saline (PBS)] for 30 min. (c) Quantification of data represented in (b), showing that α‐BTX interferes with binding of the α7 nAChR subunit to Lypd6‐coupled beads (n = 5). Values are normalized to their individual vehicle and represented as mean ± SEM. **p < 0.01 indicates statistical difference from affinity purification in the absence of α‐BTX in a ratio paired t‐test.