REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Mouse monoclonal anti-GFP (JL-8, for western blot) | Clontech | Cat. #632381; RRID:AB_2313808 |
Rabbit polyclonal anti-GFP (for immuno-EM) | Santa Cruz | Cat. #sc-8334; RRID:AB_641123 |
Mouse monoclonal anti-HA (16B12) | Biolegend | Cat. #901502; RRID:AB_2565007 |
Alpaca anti-GFP coupled to agarose beads | Chromotek | Cat. #GFP-Trap®_A |
Pierce® Anti-HA Agarose | Thermofisher | Cat. #26181; RRID:AB_2537081 |
Chemicals, Peptides, and Recombinant Proteins | ||
Dexamethasone | Sigma-Aldrich | Cat. #D1756–25MG; CAS: 50-02-2 |
DAPI | Sigma-Aldrich | Cat. #D9542-5MG; CAS: 28718-90-3 |
LR Clonase® II Plus enzyme | Thermofisher | Cat. #12538120 |
Protease inhibitor cocktail | Sigma-Aldrich | Cat. #P9599-5ML; EC: 200-664-3 |
TRIzol Reagent | Thermofisher | Cat. #15596026 |
Critical Commercial Assays | ||
QuikChange II site-directed mutagenesis kit | Agilent | Cat. #200524 |
Wheat germ in vitro translation system | BioSieg | N/A |
SuperScript® III First-Strand Synthesis System | Thermofisher | Cat. #18080050 |
FastStart Universal SYBR Green Master Kit | Roche | Cat. # 04913850001 |
Deposited Data | ||
Microarray raw and analyzed data | This paper | GEO: GSE72742, GSE72743 |
Experimental Models: Cell Lines | ||
Experimental Models: Organisms/Strains | ||
Arabidopsis: 35S::GFP-CPR5 | Wang et al., 2014 | N/A |
Arabidopsis: 35S::YFP-CPR5/G120D | This paper | N/A |
Arabidopsis: 35S/Dex::YFP-CPR5-C | This paper | N/A |
Arabidopsis: 35S::(nYFP-CPR5+cYFP-CPR5) | This paper | N/A |
Arabidopsis: 35S::(GFP-CPR5+3HA-CPR5) | This paper | N/A |
Arabidopsis: GFP-WIP1 | The Meier Laboratory | N/A |
Arabidopsis: Nup155-YFP | This paper | N/A |
Arabidopsis: MOS7-GFP | Cheng et al., 2009 | N/A |
nup88 (mos7-1), nup96 (mos3-1), importin-α3 (mos6-2) | The Li Laboratory, Cheng et al., 2009; Zhang and Li, 2005; Palma et al., 2005 |
N/A |
All other nucleoporin and transport receptor mutants Used in this study, see Table S2 |
Arabidopsis Biological Resource Center |
see Table S2 for Salk line ID |
Arabidopsis: 35S::YFP-SIM | This paper | N/A |
Arabidopsis: Dex::AvrRpt2 | McNellis et al., 1998 | N/A |
Recombinant DNA | ||
pMDC43-GFP-CPR5 | Wang et al., 2014 | N/A |
pEG100-YFP-CPR5 (WT and mutant constructs) | This paper | N/A |
pEG100-n/cYFP-CPR5 | This paper | N/A |
pEG100-Nups-YFP/cYFP | This paper | N/A |
pBAV154/pEG100-YFP-CPR5-C | This paper | N/A |
pEG100-NPR1/JAZ1/ABI5-mCherry | This paper | N/A |
pEG100-mCherry-WIT1 | This paper | N/A |
Sequence-Based Reagents | ||
Primers used in this study, see Table S3 | This paper | N/A |
Software and Algorithms | ||
Mascot (v2.5.0) | Matrix Science | N/A |
Scaffold (v4.4.1.1) | Proteome Software, Inc. |
N/A |
R (v3.0.1) | N/A | N/A |
GeneSpring (v13.0) | Agilent | N/A |
MASTA | Reina-Pinto et al., 2010 | N/A |
PlantGSEA | Yi et al., 2013 | http://structuralbiology.cau.edu.cn/PlantGSEA/ |
Athena | O’Connor et al., 2005 | http://www.bioinformatics2.wsu.edu/cgi-bin/Athena/cgi/home.pl |
Other | ||
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
Antibodies | ||
Mouse monoclonal anti-GFP (JL-8, for western blot) | Clontech | Cat. #632381; RRID:AB_2313808 |
Rabbit polyclonal anti-GFP (for immuno-EM) | Santa Cruz | Cat. #sc-8334; RRID:AB_641123 |
Mouse monoclonal anti-HA (16B12) | Biolegend | Cat. #901502; RRID:AB_2565007 |
Alpaca anti-GFP coupled to agarose beads | Chromotek | Cat. #GFP-Trap®_A |
Pierce® Anti-HA Agarose | Thermofisher | Cat. #26181; RRID:AB_2537081 |
Chemicals, Peptides, and Recombinant Proteins | ||
Dexamethasone | Sigma-Aldrich | Cat. #D1756-25MG; CAS: 50-02-2 |
DAPI | Sigma-Aldrich | Cat. #D9542-5MG; CAS: 28718-90-3 |
LR Clonase® II Plus enzyme | Thermofisher | Cat. #12538120 |
Protease inhibitor cocktail | Sigma-Aldrich | Cat. #P9599-5ML; EC: 200-664-3 |
TRIzol Reagent | Thermofisher | Cat. #15596026 |
Critical Commercial Assays | ||
QuikChange II site-directed mutagenesis kit | Agilent | Cat. #200524 |
Wheat germ in vitro translation system | BioSieg | N/A |
SuperScript® III First-Strand Synthesis System | Thermofisher | Cat. #18080050 |
FastStart Universal SYBR Green Master Kit | Roche | Cat. # 04913850001 |
Deposited Data | ||
Microarray raw and analyzed data | This paper | GEO: GSE72742, GSE72743 |
Experimental Models: Cell Lines | ||
Experimental Models: Organisms/Strains | ||
Arabidopsis: 35S::GFP-CPR5 | Wang et al., 2014 | N/A |
Arabidopsis: 35S::YFP-CPR5/G120D | This paper | N/A |
Arabidopsis: 35S/Dex::YFP-CPR5-C | This paper | N/A |
Arabidopsis: 35S::(nYFP-CPR5+cYFP-CPR5) | This paper | N/A |
Arabidopsis: 35S::(GFP-CPR5+3HA-CPR5) | This paper | N/A |
Arabidopsis: GFP-WIP1 | The Meier Laboratory | N/A |
Arabidopsis: Nup155-YFP | This paper | N/A |
Arabidopsis: MOS7-GFP | Cheng et al., 2009 | N/A |
nup88 (mos7-1), nup96 (mos3-1), importin-α3 (mos6-2) | The Li Laboratory, Cheng et al., 2009; Palma et al., 2005; Zhang and Li, 2005 |
N/A |
All other nucleoporin and transport receptor mutants Used in this study, see Table S2 |
Arabidopsis Biological Resource Center |
see Table S2 for Salk line ID |
Arabidopsis: 35S::YFP-SIM | This paper | N/A |
Arabidopsis: Dex::AvrRpt2 | McNellis et al., 1998 | N/A |
Recombinant DNA | ||
pMDC43-GFP-CPR5 | Wang et al., 2014 | N/A |
pEG100-YFP-CPR5 (WT and mutant constructs) | This paper | N/A |
pEG100-n/cYFP-CPR5 | This paper | N/A |
pEG100-Nups-YFP/cYFP | This paper | N/A |
pBAV154/pEG100-YFP-CPR5-C | This paper | N/A |
pEG100-NPR1/JAZ1/ABI5-mCherry | This paper | N/A |
pEG100-mCherry-WIT1 | This paper | N/A |
Sequence-Based Reagents | ||
Primers used in this study, see Table S3 | This paper | N/A |
Software and Algorithms | ||
Mascot (v2.5.0) | Matrix Science | N/A |
Scaffold (v4.4.1.1) | Proteome Software, Inc. |
N/A |
R (v3.0.1) | N/A | N/A |
GeneSpring (v13.0) | Agilent | N/A |
MASTA | Reina-Pinto et al., 2010 | N/A |
PlantGSEA | Yi et al., 2013 | http://structuralbiology.cau.edu.cn/PlantGSEA/ |
Athena | O’Connor et al., 2005 | http://www.bioinformatics2.wsu.edu/cgi-bin/Athena/cgi/home.pl |
Other |