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. Author manuscript; available in PMC: 2017 Sep 8.
Published in final edited form as: Cell. 2016 Aug 25;166(6):1526–1538.e11. doi: 10.1016/j.cell.2016.07.042

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Mouse monoclonal anti-GFP (JL-8, for western blot) Clontech Cat. #632381;
RRID:AB_2313808
Rabbit polyclonal anti-GFP (for immuno-EM) Santa Cruz Cat. #sc-8334;
RRID:AB_641123
Mouse monoclonal anti-HA (16B12) Biolegend Cat. #901502;
RRID:AB_2565007
Alpaca anti-GFP coupled to agarose beads Chromotek Cat. #GFP-Trap®_A
Pierce® Anti-HA Agarose Thermofisher Cat. #26181;
RRID:AB_2537081
Chemicals, Peptides, and Recombinant Proteins
Dexamethasone Sigma-Aldrich Cat. #D1756–25MG;
CAS: 50-02-2
DAPI Sigma-Aldrich Cat. #D9542-5MG;
CAS: 28718-90-3
LR Clonase® II Plus enzyme Thermofisher Cat. #12538120
Protease inhibitor cocktail Sigma-Aldrich Cat. #P9599-5ML;
EC: 200-664-3
TRIzol Reagent Thermofisher Cat. #15596026
Critical Commercial Assays
QuikChange II site-directed mutagenesis kit Agilent Cat. #200524
Wheat germ in vitro translation system BioSieg N/A
SuperScript® III First-Strand Synthesis System Thermofisher Cat. #18080050
FastStart Universal SYBR Green Master Kit Roche Cat. # 04913850001
Deposited Data
Microarray raw and analyzed data This paper GEO: GSE72742,
GSE72743
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
Arabidopsis: 35S::GFP-CPR5 Wang et al., 2014 N/A
Arabidopsis: 35S::YFP-CPR5/G120D This paper N/A
Arabidopsis: 35S/Dex::YFP-CPR5-C This paper N/A
Arabidopsis: 35S::(nYFP-CPR5+cYFP-CPR5) This paper N/A
Arabidopsis: 35S::(GFP-CPR5+3HA-CPR5) This paper N/A
Arabidopsis: GFP-WIP1 The Meier Laboratory N/A
Arabidopsis: Nup155-YFP This paper N/A
Arabidopsis: MOS7-GFP Cheng et al., 2009 N/A
nup88 (mos7-1), nup96 (mos3-1), importin-α3 (mos6-2) The Li Laboratory,
Cheng et al., 2009;
Zhang and Li, 2005;
Palma et al., 2005 		N/A
All other nucleoporin and transport receptor mutants
Used in this study, see Table S2 		Arabidopsis Biological
Resource Center		 see Table S2 for
Salk line ID
Arabidopsis: 35S::YFP-SIM This paper N/A
Arabidopsis: Dex::AvrRpt2 McNellis et al., 1998 N/A
Recombinant DNA
pMDC43-GFP-CPR5 Wang et al., 2014 N/A
pEG100-YFP-CPR5 (WT and mutant constructs) This paper N/A
pEG100-n/cYFP-CPR5 This paper N/A
pEG100-Nups-YFP/cYFP This paper N/A
pBAV154/pEG100-YFP-CPR5-C This paper N/A
pEG100-NPR1/JAZ1/ABI5-mCherry This paper N/A
pEG100-mCherry-WIT1 This paper N/A
Sequence-Based Reagents
Primers used in this study, see Table S3 This paper N/A
Software and Algorithms
Mascot (v2.5.0) Matrix Science N/A
Scaffold (v4.4.1.1) Proteome Software,
Inc.		 N/A
R (v3.0.1) N/A N/A
GeneSpring (v13.0) Agilent N/A
MASTA Reina-Pinto et al., 2010 N/A
PlantGSEA Yi et al., 2013 http://structuralbiology.cau.edu.cn/PlantGSEA/
Athena O’Connor et al., 2005 http://www.bioinformatics2.wsu.edu/cgi-bin/Athena/cgi/home.pl
Other
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Mouse monoclonal anti-GFP (JL-8, for western blot) Clontech Cat. #632381;
RRID:AB_2313808
Rabbit polyclonal anti-GFP (for immuno-EM) Santa Cruz Cat. #sc-8334;
RRID:AB_641123
Mouse monoclonal anti-HA (16B12) Biolegend Cat. #901502;
RRID:AB_2565007
Alpaca anti-GFP coupled to agarose beads Chromotek Cat. #GFP-Trap®_A
Pierce® Anti-HA Agarose Thermofisher Cat. #26181;
RRID:AB_2537081
Chemicals, Peptides, and Recombinant Proteins
Dexamethasone Sigma-Aldrich Cat. #D1756-25MG;
CAS: 50-02-2
DAPI Sigma-Aldrich Cat. #D9542-5MG;
CAS: 28718-90-3
LR Clonase® II Plus enzyme Thermofisher Cat. #12538120
Protease inhibitor cocktail Sigma-Aldrich Cat. #P9599-5ML;
EC: 200-664-3
TRIzol Reagent Thermofisher Cat. #15596026
Critical Commercial Assays
QuikChange II site-directed mutagenesis kit Agilent Cat. #200524
Wheat germ in vitro translation system BioSieg N/A
SuperScript® III First-Strand Synthesis System Thermofisher Cat. #18080050
FastStart Universal SYBR Green Master Kit Roche Cat. # 04913850001
Deposited Data
Microarray raw and analyzed data This paper GEO: GSE72742,
GSE72743
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
Arabidopsis: 35S::GFP-CPR5 Wang et al., 2014 N/A
Arabidopsis: 35S::YFP-CPR5/G120D This paper N/A
Arabidopsis: 35S/Dex::YFP-CPR5-C This paper N/A
Arabidopsis: 35S::(nYFP-CPR5+cYFP-CPR5) This paper N/A
Arabidopsis: 35S::(GFP-CPR5+3HA-CPR5) This paper N/A
Arabidopsis: GFP-WIP1 The Meier Laboratory N/A
Arabidopsis: Nup155-YFP This paper N/A
Arabidopsis: MOS7-GFP Cheng et al., 2009 N/A
nup88 (mos7-1), nup96 (mos3-1), importin-α3 (mos6-2) The Li Laboratory,
Cheng et al., 2009;
Palma et al., 2005;
Zhang and Li, 2005 		N/A
All other nucleoporin and transport receptor mutants
Used in this study, see Table S2 		Arabidopsis Biological
Resource Center		 see Table S2 for
Salk line ID
Arabidopsis: 35S::YFP-SIM This paper N/A
Arabidopsis: Dex::AvrRpt2 McNellis et al., 1998 N/A
Recombinant DNA
pMDC43-GFP-CPR5 Wang et al., 2014 N/A
pEG100-YFP-CPR5 (WT and mutant constructs) This paper N/A
pEG100-n/cYFP-CPR5 This paper N/A
pEG100-Nups-YFP/cYFP This paper N/A
pBAV154/pEG100-YFP-CPR5-C This paper N/A
pEG100-NPR1/JAZ1/ABI5-mCherry This paper N/A
pEG100-mCherry-WIT1 This paper N/A
Sequence-Based Reagents
Primers used in this study, see Table S3 This paper N/A
Software and Algorithms
Mascot (v2.5.0) Matrix Science N/A
Scaffold (v4.4.1.1) Proteome Software,
Inc.		 N/A
R (v3.0.1) N/A N/A
GeneSpring (v13.0) Agilent N/A
MASTA Reina-Pinto et al., 2010 N/A
PlantGSEA Yi et al., 2013 http://structuralbiology.cau.edu.cn/PlantGSEA/
Athena O’Connor et al., 2005 http://www.bioinformatics2.wsu.edu/cgi-bin/Athena/cgi/home.pl
Other