KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| InVivoMAb Anti-mouse TCR Vγ2 | BioXCell | Clone:UC3-106 Cat#: BE0168 |
| InVivoMAb Anti-mouse PD-L1 | BioXCell | Clone: 10F.9G2 Cat#: BE0101 |
| InVivoMAb Anti-mouse Galectin-9 | BioXCell | Clone: RG9-1 Cat#: BE0218 |
| InVivoMAb Anti-mouse CD4 | BioXCell | Clone: GK1.5 Cat#: BE0003-1 |
| InVivoMAb Anti-Mouse CD8 | Monoclonal Antibody Core Facility, Sloan Kettering Institute |
Clone: 53-6.72 |
| WB: Rabbit mAb Bcl-XL | Cell Signalling | Clone: 54H6 |
| WB: Rabbit mAb Rb | Cell Signalling | Clone: D20 |
| WB: Rabbit mAb c-Myc | Cell Signalling | Clone: D84C12 |
| WB: Mouse mAb PTEN | Cell Signalling | Clone: 26H9 |
| WB: Mouse mAb p53 | Cell Signalling | Clone: 1C12 |
| WB: Mouse mAb β-actin | Cell Signalling | Clone: 8H10D10 |
| IHC: Anti-mouse CD4 | BD Biosciences | Clone: RM4-5 Cat#: 550280 |
| IHC: Anti-mouse CD8 | Abcam | Clone: YTS169.4 Cat#: ab22378 |
| IHC: Anti-mouse GFP | Cell Signalling | Clone: D5.1 Cat#: 2956 |
| IHC: Anti-human γ/δ TCR | Biolegend | Clone: B1 |
| Purified anti-mouse CD3ε | Biolegend | Clone: 145-2C11 |
| Purified anti-mouse CD28 | Biolegend | Clone: 37.51 |
| FC: anti-mouse CD3 | Biolegend | Clone: 17A2 |
| FC: anti-mouse CD4 | Biolegend | Clone: RM4-5 |
| FC: anti-mouse CD8 | Biolegend | Clone: 53-6.7 |
| FC/IF: anti-mouse γ/δ TCR | Biolegend | Clone: GL3 |
| FC: anti-mouse CD62L | Biolegend | Clone: MEL-14 |
| FC: anti-mouse FasL | Biolegend | Clone: MFL3 |
| FC: anti-mouse Vγ1 | Biolegend | Clone: 2.11 |
| FC: anti-mouse Vγ2(4) | Biolegend | Clone: UC-10A6 |
| FC: anti-mouse NK1.1 | Biolegend | Clone: PK136 |
| FC: anti-mouse CD39 | Biolegend | Clone: Duha59 |
| FC: anti-mouse CCR2 | Biolegend | Clone: SA203G11 |
| FC: anti-mouse CCR5 | Biolegend | Clone: HM-CCR5 |
| FC: anti-mouse CCR6 | Biolegend | Clone: 29-2L17 |
| FC: anti-mouse CD44 | Biolegend | Clone: IM7 |
| FC: anti-mouse JAML | Biolegend | Clone: 4E10 |
| FC: anti-mouse NKG2D | Biolegend | Clone: CX5 |
| FC/IF: anti-mouse/human CD11b | Biolegend | Clone: M1/70 |
| FC: anti-mouse Gr1 | Biolegend | Clone: RB6-8C5 |
| FC: anti-mouse PD-1 | Biolegend | Clone: 29F.1A12 |
| FC: anti-mouse ICOS | Biolegend | Clone: 15F9 |
| FC/IF: anti-mouse αβ TCR | Biolegend | Clone: H57-597 |
| FC: anti-mouse TLR4 | Biolegend | Clone: SA15-21 |
| FC: anti-mouse TNF-α | Biolegend | Clone: MP6-XT22 |
| FC: anti-mouse IL-17 | Biolegend | Clone: TC11-1810.1 |
| FC: anti-mouse IL-10 | Biolegend | Clone: JES5-16E3 |
| FC: anti-mouse INF-γ | Biolegend | Clone: XMG1.2 |
| FC: anti-mouse PD-L1 | Biolegend | Clone: 10F.9G2 |
| FC: anti-mouse Galectin 9 | Biolegend | Clone: RG9-35 |
| FC: anti-mouse B7-1 | Biolegend | Clone: 16-10A1 |
| FC: anti-mouse B7-2 | Biolegend | Clone: PO3 |
| FC: anti-mouse ICOSL | Biolegend | Clone: HK5.3 |
| FC: anti-mouse OX-40L | Biolegend | Clone: RM134L |
| FC: anti-mouse CD107a | Biolegend | Clone: 1D4B |
| FC: anti-mouse OX40 | Biolegend | Clone: OX-86 |
| FC: anti-mouse CTLA4 | Biolegend | Clone: UC10-4B9 |
| FC: anti-mouse Ly6C | Biolegend | Clone: HK1.4 |
| FC: anti-mouse Ly6G | Biolegend | Clone: 1A8 |
| FC: anti-mouse TLR7 | Imgenex | Clone: IMG-581A |
| FC: anti-mouse TLR9 | Imgenex | Clone: 26C593.2 |
| FC: anti-mouse Tbet | eBioscience | Clone: eBio4B10 |
| FC: anti-mouse IL-13 | eBioscience | Clone: eBio13A |
| FC: anti-mouse Granzyme B | eBioscience | Clone: NGZB |
| FC: anti-mouse GATA-3 | eBioscience | Clone: TWAJ |
| FC: anti-mouse FoxP3 | eBioscience | Clone: FJK-16s |
| FC: H-2kb SINFEKL OVA Pentamer | ProImmune | Cat#: F093 |
| FC: anti-human CD45 | Biolegend | Clone: HI30 |
| FC: anti-human CD3 | Biolegend | Clone: SK7 |
| FC: anti-human CD45RA | Biolegend | Clone: HI100 |
| FC: anti-human CD27 | Biolegend | Clone: O323 |
| FC: anti-human CD62L | Biolegend | Clone: DREG-56 |
| FC: anti-human CD14 | Biolegend | Clone: HCD14 |
| FC: anti-human CD15 | Biolegend | Clone: W6D3 |
| FC: anti-human CD11c | Biolegend | Clone: 3.9 |
| FC: anti-human Vγ9 | Biolegend | Clone: B3 |
| FC: anti-human PD-L1 | Biolegend | Clone: 29E.2A3 |
| FC: anti-human Galectin 9 | Biolegend | Clone: 9M1-3 |
| FC/IF: anti-human γ/δ TCR | eBioscience | Clone: B1.1 |
| IF: anti-human αβ TCR | Biolegend | Clone: IP26 |
| IF: anti-mouse PD-L1 | Abcam | Cat# ab58810 |
| IF: anti-mouse CK-19 | Developmental Studies Hybridoma Bank, University of Iowa |
Clone: TROMA-III |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Caerulein | Sigma-Aldrich | Cat#: C9026 |
| CFSE | eBioscience | Cat#: 65-0850-84 |
| Evans Blue | Sigma-Aldrich | Cat#: E2129 |
| Critical Commercial Assays | ||
| Lipase Basic Kit | Sigma-Aldrich | Cat#: 626327 |
| Amylase Activity Kit | Sigma-Aldrich | Cat#: MAK009 |
| Cytometric Bead Array Mouse Inflammation Kit | BD Biosciences | Cat#: 52364 |
| Cytometric Bead Array Mouse Th1/Th2 Cytokine Kit | BD Biosciences | Cat#: 551287 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Mouse: KPC Pancreatic Cancer Cell line | Laboratory of Dave Tuveson | Cell line: FC1242 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6 (H-2Kb) | The Jackson Laboratory | JAX: 000664 |
| Mouse: C57BL/6-Trdctm1Mal | The Jackson Laboratory | JAX: 016941 |
| Mouse: CCR2−/− | The Jackson Laboratory | JAX: 004999 |
| Mouse: CCR5−/− | The Jackson Laboratory | JAX: 005427 |
| Mouse: CCL2−/− | The Jackson Laboratory | JAX: 004434 |
| Mouse: CCR6−/− | The Jackson Laboratory | JAX: 005793 |
| Mouse: B6.129P2-Tcrdtm1Mom/J (Tcrδ−/−) | The Jackson Laboratory | JAX: 002120 |
| Mouse: B6. LSL-KrasG12D and p48Cre (KC ) | Laboratory of Dafna Bar- Sagi |
|
| Recombinant DNA | ||
| Plasmid: pCI-neo-cOVA | Addgene | Plasmid # 25097 |
| Sequence-Based Reagents | ||
| Software and Algorithms | ||
| Other | ||