Figure 3.

Transfer of Fuc-liposome-Cy5.5 into colorectal cancer (CRC) cell lines. A) Transfer of Fuc-liposome-Cy5.5 into CRC cells. Colo205 cells were incubated with Fuc-liposome-Cy5.5 for two hours, then washed twice with phosphate-buffered saline and visualized by fluorescence microscopy. NT = no treatment: F0, F0-liposome-Cy5.5: F25, F25-liposome-Cy5.5: F50, F50-liposome-Cy5.5: F50 + FUC, F50-liposome-Cy5.5 with excess L-fucose. Scale bar = 50 µm. B) Flow cytometric analysis of Fuc- liposome-Cy5.5-treated cells. CRC cells were treated with Fuc-liposome-Cy5.5 for two hours with or without excess of L-fucose and were analyzed by flow cytometry. Percent of Cy5.5-positive cells was shown. NT = no treatment: F0, F0-liposome-Cy5.5: F25, F25-liposome-Cy5.5: F50, F50-liposome-Cy5.5: F50 + FUC, F50-liposome-Cy5.5 with excess L-fucose. The experiments were performed in triplicate and repeated twice. Statistical analysis was performed by χ² test (two-sided). *P < .05.