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. 2016 Sep 9;5(1):1521. doi: 10.1186/s40064-016-3037-0

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Flow cytometry analysis to determine gene expression changes. Histograms of raw data were divided to determine the number of GFP negative (left side) and GFP positive (right side) cells on the plot for each sample. The number of cells expressing GFP did not decrease in control samples (a–d). Side-by-side, experimental samples exhibited a significant increase in GFP negative cells when injected three times (LANx3) compared to cells injected once (LANx1) in 1.5 mA injections (e, f), 4.5 mA injections (g, h), and 6.0 mA injections (i, j). Compare to data in Table 1 and Fig. 4