Table 2.

One-sided T test results from comparisons of multiple (x3) versus single (x1) injected samples

Multiple injections (1) versus single injections (2)	P value	Difference in mean GFP KO (1–2) (%)
1.5 mA, x3 versus 1.5 mA, x1	<0.0001	59.87
4.5 mA, x3 versus 4.5 mA, x1	<0.0001	57.93
6.0 mA, x3 versus 6.0 mA, x1	<0.0001	47.36

Represented data was initially screened in JMP using ANOVA test to determine presence of statistically significance relationships followed by one-sided t test (α = 0.05) evaluation of specific comparisons. Default minimum p value reported is 0.0001. All represented relationships are statistically different