Table 3.
Identified protein spots differentiating the studied classes: C(8) and Tu + (8), C(8) and D + (8), C(8) and Tu + D(8), C(8) and C(9), C(9) and Tu − (9) as well as Tu + (8) and Tu − (9) in multivariate analysis at P ≤ 0.05 (see Table 2)
| Protein spot | Protein name (against NBCI database) | Accession | Score | MW/pIt (kDa 103) | Matches | Sequences | Coverage | Biological relevance | |
|---|---|---|---|---|---|---|---|---|---|
| No. | ↓/↑ | ||||||||
| C(8) and Tu + (8) | |||||||||
| 2 | ↓ | Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) partial [Sorghastrum nutans] | gi|375493221 | 2437 | 49.92/6.44 | 103 (103) | 17 (17) | 60 | Photosynthesis related |
| 10 | ↓ | Putative TCP-1/cpn60 chaperonin family protein partial [Zea mays] (cpn60) | gi|413942615 | 1459 | 35.72/5.09 | 40 (40) | 18 (18) | 60 | Chaperone protein |
| 11 | ↑ | Oxygen evolving enhancer protein3 containing protein [Zea mays] (OEE3) | gi|195609634 | 1668 | 25.91/7.66 | 52 (52) | 8 (8) | 43 | Photosynthesis related |
| 12 | ↑ | Heat shock cognate 70 kDa protein2 [Zea mays] (HSC70) | gi|293334615 | 1184 | 71.52/5.13 | 36 (36) | 24 (24) | 41 | Chaperone protein |
| C(8) and D + (8) | |||||||||
| 1 | ↑ | RuBisCO, large subunit, partial (chloroplast) [Campanula trache] | gi|253992223 | 451 | 50.52/6.46 | 20 (20) | 9 (9) | 15 | Photosynthesis related |
| 3 | ↑ | RuBisCO, small subunit2 [Zea mays] | gi|226532904 | 741 | 18.19/8.19 | 28 (28) | 9 (9) | 64 | Photosynthesis related |
| 4 | ↓ | Drought-inducible 22 kDa protein [Saccharum officinarum] | gi|15667623 | 174 | 15.92/7.78 | 5 (5) | 4 (4) | 35 | Stress responsive |
| 5 | ↓ | Plastid ADP-glucose pyrophosphorylase large subunit (AGPase) [Zea mays] | gi|162460455 | 1203 | 55.50/8.57 | 26 (26) | 9 (9) | 24 | Starch biosynthesis |
| 8 | ↓ | Chloroplast protein synthesis2 [Zea mays] (cps2) | gi|413945149 | 501 | 63.26/5.06 | 6 (6) | 5 (5) | 13 | Photosynthesis related |
| 11 | ↑ | Glyoxylase1 [Zea mays] | gi|162461576 | 949 | 32.45/5.39 | 30 (30) | 13 (13) | 53 | Glyoxalase system |
| 12 | ↑ | Unknown [Zea mays] | gi|194688752 | 4449 | 47.67/5.95 | 133 (133) | 19 (19) | 51 | Not identified |
| 13 | ↑ | 17.5 kDa class II heat shock protein [Zea mays] | gi|195639038 | 878 | 17.80/5.17 | 33 (33) | 4 (4) | 32 | Heat shock protein |
| 14 | ↑ | LOC100192117 [Zea mays] (PR-10) | gi|212275926 | 1466 | 17.05/5.38 | 41 (41) | 12 (12) | 86 | Pathogenesis-related protein |
| 17 | ↑ | cpn60 | gi|413942615 | 1459 | 35.73/5.09 | 40 (40) | 18 (18) | 60 | Chaperone protein |
| 18 | ↓ | Unknown [Zea mays] | gi|223948025 | 2695 | 61.97/5.42 | 71 (71) | 28 (28) | 56 | Not identified |
| 20 | ↑ | NADP-malic enzyme [Zea mays] (NADP-ME) | gi|30575690 | 822 | 70.41/6.20 | 23 (23) | 6 (6) | 29 | Photosynthesis related |
| 21 | ↓ | RuBisCO large subunit-binding protein subunit α, LOC100281701 [Zea mays] | gi|226493235 | 4487 | 61.1/5.20 | 107 (107) | 21 (21) | 64 | Chaperone protein |
| C(8) and Tu + D(8) | |||||||||
| 23 | ↑ | Phosphoenolpyruvate carboxylase (PEPC) [Zea mays] | gi|27764449 | 389 | 109.85/5.7 | 21 (21) | 19 (19) | 22 | Photosynthesis related |
| 3 | ↑ | Pyruvate, orthophosphate dikinase (PPDK) [Zea mays] | gi|168586 | 1589 | 103.36/5.7 | 41 (41) | 18 (18) | 27 | Photosynthesis related |
| 8 | ↓ | Peptidyl-prolyl cis–trans isomerase family protein isoform1 [Zea mays] (PPIases) | gi|212723348 | 309 | 35.3/8.66 | 9 (9) | 8 (8) | 29 | Chaperone protein |
| 13 | ↓ | cps2 | gi|413945149 | 501 | 63.26/5.06 | 6 (6) | 5 (5) | 13 | Photosynthesis related |
| 5 | ↑ | β-d-glucosidase precursor [Zea mays] | gi|343227637 | 1895 | 63.46/6.75 | 64 (64) | 18 (18) | 30 | O-glycosyl compounds hydrolysis |
| 6 | ↑ | Drought-inducible 22 kDa protein [Saccharum officinarum] | gi|15667623 | 174 | 15.92/5.78 | 5 (5) | 4 (4) | 35 | Stress responsive |
| 14 | ↑ | Aspartate aminotransferase (AAT) [Zea mays] | gi|226508814 | 379 | 50.55/8.15 | 12 (12) | 11 (11) | 24 | Amino acids biosynthesis |
| 16 | ↑ | PPDK [Zea mays] | gi|168586 | 5109 | 103.3/5.71 | 149 (149) | 30 (30) | 42 | Photosynthesis related |
| 24 | ↑ | Cytosolic PPDK [Zea mays subsp. mays] | gi|238928442 | 1949 | 96.43/5.42 | 46 (46) | 11 (11) | 34 | Photosynthesis related |
| 26 | ↑ | Predicted stromal 70 kDa heat shock-related protein chloroplastic-like [Brachypodium distachyon] | gi|357134135 | 1194 | 73.20/5.04 | 37 (37) | 15 (15) | 25 | Heat shock protein |
| 15 | ↑ | Unknown [Zea mays] | gi|223949895 | 3742 | 41.47/4.91 | 88 (88) | 21 (21) | 57 | Not identified |
| 4 | ↓ | Unknown [Zea mays] | gi|223949895 | 692 | 41.47/4.91 | 11 (11) | 5 (5) | 16 | Not identified |
| C(8) and C(9) | |||||||||
| 2 | ↑ | ATP synthase CF1 α subunit [Chasmanthium latifolium] (atpA) | gi|307697215 | 1937 | 55.69/5.73 | 74 (74) | 21 (21) | 44 | Energy metabolism |
| 10 | ↓ | PPDK [Zea mays] | gi|168586 | 4547 | 103.36/5.7 | 138 (138) | 32 (32) | 46 | Photosynthesis related |
| 5 | ↓ | Fructose-bisphosphate aldolase [Zea mays] | gi|195634659 | 3949 | 41.92/7.63 | 150 (150) | 18 (18) | 49 | Glycolysis/gluconeogenesis |
| 1 | ↓ | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cytosolic2 [Zea mays] | gi|162461501 | 1110 | 36.63/6.41 | 36 (36) | 13 (13) | 42 | Glycolysis |
| C(9) and Tu − (9) | |||||||||
| 5 | ↑ | atpA | gi|307697215 | 1937 | 55.69/5.73 | 74 (74) | 21 (21) | 44 | Energy metabolism |
| 13 | ↓ | PPDK [Zea mays] | gi|168586 | 4317 | 103.36/5.7 | 135 (135) | 36 (36) | 47 | Photosynthesis related |
| 1 | ↓ | GAPDH cytosolic2 | gi|162461501 | 1110 | 36.63/6.41 | 36 (36) | 13 (13) | 42 | Glycolysis |
| 2 | ↓ | GAPDH partial [Zea mays] | gi|293889 | 1064 | 26.49/6.25 | 33 (33) | 12 (12) | 61 | Glycolysis |
| 6 | ↓ | Fructose-bisphosphate aldolase [Zea mays] | gi|195634659 | 3949 | 41.92/7.63 | 150 (150) | 18 (18) | 49 | Glycolysis/gluconeogenesis |
| Tu + (8) and Tu − (9) | |||||||||
| 1 | ↓ | GAPDH cytosolic2 [Zea mays] | gi|162461501 | 1110 | 36633 | 36 (36) | 13 (13) | 42 | Glycolysis |
| 3 | ↓ | Fructose-bisphosphate aldolase [Zea mays] | gi|195634659 | 518 | 41.92/7.63 | 16 (16) | 11 (11) | 36 | Glycolysis/gluconeogenesis |
| 5 | ↑ | Superoxide dismutase [Mn] 3.4 mitochondrial precursor [Zea mays] | gi|212722004 | 367 | 25.21/6.71 | 15 (15) | 7 (7) | 38 | Antioxidant activity |
| 6 | ↑ | RuBisCO large subunit-binding protein subunit β [Zea mays] | gi|195630027 | 942 | 64.4/5.81 | 26 (26) | 16 (16) | 29 | Chaperone protein |
| 8 | ↑ | Fructose-bisphosphate aldolase [Zea mays] | gi|195634659 | 1277 | 41.92/7.63 | 36 (36) | 12 (12) | 40 | Glycolysis/gluconeogenesis |
Upward arrow (↑) indicates up-regulating effect
Downward arrow (↓) indicates down-regulating effect