Figure 5.

Stress-induced Src phosphorylation and cell migration can be blunted by dasatinib.

(A) Western blot for pY416Src and GAPDH loading control in CHLA10, CHLA25 and A673 cells upon application of no stress (NS) or stress (S, hypoxia and serum deprivation) conditions. (B) Western blot for pY416Src and GAPDH loading control in stressed cells treated with either DMSO (DM) or 50 nM dasatinib (DAS). Numbers listed under the blots in (A-B) correspond to normalized values of band pixel intensity per cell line examined. (C) Cells under stress conditions were treated with DMSO or 50 nM dasatinib and subjected to xCELLigence migration assays. Images are representative of experiments performed at least in triplicate. The variation in the y-axis is due to the inherent differences in the ability of the different cells lines to migrate at baseline or under stress conditions.