Figure 3.

Two independent methods to perturb cell diameter. (A) Cells expressing MreB^msfGFP were grown to steady state in different concentrations of the polymerization-inhibiting drug A22. All treatments are below the lethal concentrations of A22. As A22 concentration increases, cells significantly increase their diameter. (B) Cell diameter can also be changed with single-point mutants in the mreB^msfGFP. Fourteen mutants were generated with diameters both larger and smaller than the unmutated form (WT). Mutants are arranged in order of increasing diameter. Error bars represent 80% confidence intervals. To see this figure in color, go online.