Table 2.

Electrophoretic Mobility and Z_DHH of Lipid Nanodiscs

Nanodisc	μ (cm2/V·s)	ZDHH·Nanodisca	ZDHH·Ca2+	ZDHH·Mg2+
POPCb	−4.2 × 10−5 ± 3.8 × 10−6	−14.1 ± 1.0	−10.3 ± 0.3	−14.8 ± 0.7
10% POPSb	−7.1 × 10−5 ± 2.9 × 10−6	−24.6 ± 0.8	−20.2 ± 0.4	−25.5 ± 0.8
30% POPSb	−1.2 × 10−4 ± 5.7 × 10−6	−38.5 ± 1.4	−29.5 ± 0.8	−37.9 ± 0.4
70% POPSb	−1.7 × 10−4 ± 3.8 × 10−6	−56.4 ± 2.4	−42.2 ± 1.0	−57.5 ± 1.0
POPCb	−3.8 × 10−5 ± 3.9 × 10−6	−18.0 ± 0.7	−12.6 ± 0.1	–
10% POPSc	−7.9 × 10−5 ± 3.9 × 10−6	−37.0 ± 2.0	−25.9 ± 0.1	–
30% POPSc	−1.2 × 10−4 ± 3.6 × 10−6	−57.2 ± 2.5	−39.4 ± 1.2	–
70% POPSc	−2.0 × 10−4 ± 5.0 × 10−6	−92.3 ± 4.1	−69.2 ± 4.5	–
DMPCb	−4.4 × 10−5 ± 9.7 × 10−7	−14.0 ± 0.5	–	–
10% DMPSb	−6.8 × 10−5 ± 4.1 × 10−6	−23.3 ± 0.9	–	–
30% DMPSb	−1.3 × 10−4 ± 5.9 × 10−6	−42.8 ± 0.6	–	–
50% DMPSb	−1.8 × 10−4 ± 6.8 × 10−6	−58.7 ± 1.5	–	–
10% POPAb	−7.5 × 10−5 ± 1.7 × 10−6	−26.2 ± 1.7	∗	−21.1 ± 0.8
30% POPAb	−1.4 × 10−4 ± 1.6 × 10−5	−45.0 ± 3.0	∗	−34.3 ± 1.6
70% POPAb	−2.1 × 10−4 ± 1.0 × 10−5	−66.6 ± 4.7	∗	−45.6 ± 2.0
10% PIP2b	−1.2 × 10−4 ± 3.6 × 10−6	−39.7 ± 1.1	−39.7 ± 1.1	∗
10% POPEb	−6.5 × 10−5 ± 5.3 × 10−6	−21.4 ± 1.5	–	–
Cardiolipinb	−2.4 × 10−4 ± 1.3 × 10−5	−77.6 ± 3.6	–	–
E. colib	−1.7 × 10−4 ± 1.5 × 10−6	−55.7 ± 0.6	∗	∗
E. colic	−1.7 × 10−4 ± 4.4 × 10−6	−79.1 ± 2.0	∗	∗

^aIn standard solvent (100 mM NaCl, 50 mM Tris, pH 7.4). Entries without values (–) indicate that no charge measurements were made; ^∗ indicates the formation of aggregates.

^bMSP1D1 Nanodiscs.

^cMSP1E3D1 Nanodiscs.