Table 3.

Z_DHH of the PL Component of MSP1D1 Nanodiscs

Nanodisc	Zcalc·Lipida	ZDHH·Lipidb	ZDHH·Lipid/Zcalc·Lipidc	Δz/ΔPLd
POPC	−	−	−	−
10POPE	−13	−7.3	0.56	−0.56
10POPS	−13	−10.5	0.81	−0.81
30POPS	−38	−24.4	0.64	−0.64
70POPS	−88	−42.3	0.48	−0.48
10POPA	−16	−12.1	0.75	−0.93
30POPA	−48	−30.9	0.64	−0.81
70POPA	−110	−52.5	0.48	−0.60
10PIP2	−39	−25.6	0.66	−2.00

^aCalculated assuming a constant number of lipids per Nanodisc. POPS Nanodiscs were assumed to have a −1 charge per lipid, POPA Nanodiscs were assumed to have a charge of −1.25 per lipid (48), and PIP₂ Nanodiscs were assumed to have a −3 charge per lipid (51).

^bCalculated assuming that the Z_DHH of POPC Nanodiscs, −14.1, is contributed entirely from the MSPs and that PC lipids contribute a Z_DHH of zero. Therefore, the values in the table are the measured Z_DHH less the MSP contribution, providing an estimate of the charge contribution solely from the lipid headgroups.

^cMeasured Z_DHH divided by the calculated charge.

^dZ_DHH contribution per lipid headgroup (Z_DHH·lipid/# of PLs).