Time courses of ΔF and presteady-state current relaxations correlate. (A) Representative ΔF recordings from labeled oocytes expressing S155C, S155C-Q178N, and S155C-R182A shown for voltage steps from –60 mV to the indicated potentials. In each case, recordings were made in the absence and presence of 100 mM Na+. (B) Comparison of main relaxation time constant from presteady-state current (τONQ) and from fitting ΔF (τONF) for the double mutants containing labeled Cys-116 as a reporter, measured in 100Na solution. Each data point is mean ± SE from up to five cells. (C) Comparison of main relaxation time constant from presteady-state current (τONQ) and from fitting ΔF (τONF) for the double mutants containing labeled Cys-155 as a reporter, measured in 100Na solution. Each data point is mean ± SE from >5 cells. To see this figure in color, go online.