Fig. 4.

NHAs were transfected with siRNA for β-catenin or Scrm-si for 72 h and mRNA for β-catenin and EAAT2, and GS was measured by qPCR and normalized to control (a). b PDAs were KD or over-expressed for β-catenin using siRNA and pABC and equivalent controls as indicated previously. At 72 h post β-catenin KD or 24 h post β-catenin over-expression, 2 mM of L-glutamic acid was added to the cell media and 30 min later glutamate in the supernatant was measured using a glutamate assay kit. Asterisks indicate a p < 0.05 relative to the respective control using Student’s t test. Error bars indicate SD