Figure 5.
Neutralization by mAbs from Immunized Mice
(A) Neutralization breadth and potency of mAbs isolated from several mice receiving the entire immunization program and screened on a 25-virus cross-clade panel of near-native (N276A) isolates.
(B) Neutralization activity of two mAbs isolated from mouse 286. Nem10 (circles) and Nem11 (squares) neutralized wild-type 191084 B7-19 virus grown in 293T cells (black) or 293S cells (blue), as well as N276A virus grown in 293T cells (red), with potency highest against N276A virus. In the bottom three panels, neutralization curves for mature VRC01, F105, and b12 are shown for comparison. Neutralization values with error bars are mean ± SD for two measurements. Best fit curves were calculated with GraphPad Prism.