REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Common lab reagents	N/A	N/A
Critical Commercial Assays
Quick & Easy E.coli gene deletion	Gene Bridges	Cat#K006
Deposited Data
F-pilus pED208 Cryo-EM map	This study	EMD-4042
F-pilus pED208 structure	This study	5LEG
F-pilus (12.5Å axial rise) Cryo-EM map	This study	EMD-4046
F-pilus (12.5Å axial rise) structure	This study	5LFB
F-pilus (13.2Å axial rise) Cryo-EM map	This study	EMD-4044
F-pilus (13.2Å axial rise) structure	This study	5LER
Experimental Models: Organisms/Strains
E.coli: JE2571 (leu thr fla pil str) harboring the pED208 plasmid	Lab of Prof. Ellen Zechner	N/A
E. coli: DH5α (F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1) harboring the pOX38-Cm plasmid	Lab of Prof. Fernando de la Cruz	N/A
f1 phage: ssDNA, filamentous	Lab of Dr. Neville Firth	N/A
HB101: auxotroph, F-, recA13, Res-, Mod-, Strr, Rifr	This study	N/A
Recombinant DNA
Plasmid: pBAD-M11 (modified)	This study	N/A
Sequence-Based Reagents
Primers for Kanamycin cassette generation, traA allele cloning and mutagenesis, see Table S1	This study	N/A
Software and Algorithms
Spider package	Frank et al., 1996	http://spider.wadsworth.org/spider_doc/spider/docs/spider.html
CTFFIND3	Mindell and Grigorieff, 2003	http://grigoriefflab.janelia.org/ctf
EMAN2	Tang et al., 2007	http://blake.bcm.tmc.edu/emanwiki/EMAN2
IHRSR	Egelman, 2000	N/A
COOT	Emsley et al., 2010	http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PHENIX	Adams et al., 2010	https://www.phenix-online.org/documentation/index.html
CHIMERA	Pettersen et al., 2004	http://www.rbvi.ucsf.edu/chimera/
Molprobity	Chen et al., 2010	http://molprobity.biochem.duke.edu/
LSQKAB	Kabsch, 1976	http://www.ccp4.ac.uk/
PDBSET	Winn et al., 2011	http://www.ccp4.ac.uk/
PYMOL	Molecular Graphics System, Version 1.8 Schrödinger, LLC	https://www.pymol.org/