Figure 3.

Levels of anti-SLA-specific IgG, IgG2a, and IgG1 in sera of BALB/c mice immunized SC, three times in 3 weeks intervals, with (ALM+QS)_ALG, (ALM)_ALG+QS, (ALM)_ALG, ALM+QS, ALM or PBS. Blood samples were collected from the mice, 3 weeks after the last booster (A) and 14 weeks after challenge (B). The SLA-specific IgG, IgG2a and IgG1 levels were assessed using ELISA method. Panel C indicates the ratio of IgG2a/IgG1 based on absorbance. The assays were performed in triplicate at 200-fold dilution for each serum sample. Values are the mean ± S.E.M