Table 2.
Relative changes in gene expression in HLE cells in response to culture in different media (n = 4). An asterisk indicates that the value is significantly different (p < 0.05) from that obtained for the corresponding donor matched controls (DMEM).
| Gene | Media | Days of culture | dCT | ddCT |
|---|---|---|---|---|
| p63 | SHEM | 7 | 5.95 + 0.28 | −0.55 + 0.28 |
| 14 | 5.3 + 0.16 | −0.93 + 0.15 | ||
| DMEM | 7 | 7.13 + 0.22 | 0.66 + 0.22 | |
| 14 | 6.97 + 0.21 | 0.5 + 0.21 | ||
|
| ||||
| Keratin 3 | SHEM | 7 | 6.72 + 1.15∗ | −9.65 + 1.16∗ |
| 14 | 3.67 + 0.82∗ | −12.74 + 0.82∗ | ||
| DMEM | 7 | 18.03 + 1.16 | 1.61 + 1.16 | |
| 14 | 14.52 + 0.29 | −1.9 + 0.29 | ||
|
| ||||
| Keratin 12 | SHEM | 7 | 4.79 + 0.49∗ | −6.46 + 0.49∗ |
| 14 | 3.03 + 0.45∗ | −8.2 + 0.45∗ | ||
| DMEM | 7 | 12.02 + 1.46 | 0.77 + 1.46 | |
| 14 | 10.88 + 2.3 | −0.36 + 2.3 | ||