Skip to main content
. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Hear Res. 2016 Jun 1;339:50–59. doi: 10.1016/j.heares.2016.05.016

Figure 2. Membrane prestin expression and charge density increase with time after induction in the Prestin-mGFP Tet-On inducible cell line.

Figure 2

A) Top panel: representative Western blot of biotinylated cell surface proteins showing a gradual increase in prestin expression after addition of the inducer, 2 μg/ml Dox. Control plates were grown for 72 hours in the absence of Dox. Bottom panel: average band intensity of prestin relative to EGFR calculated from three independent western blots as a function of time post-induction. B) A corresponding increase is seen in the average charge density up to 56 hours post induction, reaching a plateau at further time points. Dox was added again to the cells at 48 hours to continue robust prestin expression. Sample size, n = 6 to 9 cells for each time point. C) Average z values increase between 16–24 hours and then remain steady through 72 hours post-induction. Sample sizes were the same as in (B). D) Dual axis plot showing parallel increases in membrane prestin expression (primary y-axis) and average charge density (secondary y-axis) as a function of time post-induction. Error bars represent standard deviation in all panels. The Student’s t-test with Holm adjustment of p-values for multiple comparisons was used to estimate statistical significance; resulting p-values are indicated in each panel.