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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Hear Res. 2016 Jun 1;339:50–59. doi: 10.1016/j.heares.2016.05.016

Figure 4. Decline in prestin expression and function after Dox removal.

Figure 4

A) Top panel: representative western blot of biotinylated cell surface proteins showing the progressive decrease in prestin expression following the removal of Dox from the cell culture media. Bottom panel: average band intensity of prestin relative to EGFR calculated from three independent Western blots as a function of time after Dox removal. B) A corresponding decrease in charge density is seen up to 48 hours after Dox removal (n= 8 to 9 cells per time point). C) Average z values did not change with time after Dox removal. Sample sizes were the same as in (B). D) Dual axis plot showing parallel decreases in membrane prestin expression (primary y-axis) and average charge density (secondary y-axis) with time after Dox removal. Error bars represent standard deviation in all panels. The Student’s t-test with Holm adjustment of p-values for multiple comparisons was used to estimate statistical significance; resulting p-values are indicated in each panel.