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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Hear Res. 2016 Jun 1;339:50–59. doi: 10.1016/j.heares.2016.05.016

Table 1.

Parameters of prestin-associated charge movement from cell lines with transient or inducible prestin expression and from murine OHCs.

HEK293 cells Charge Density (fC/pF) Vpkc (mV) z n
Transient transfection 28.7 ± 9.6 −72 ± 18.9 0.8 ± 0.04 5
Dox-Induced (6 μg/ml) 12.6 ± 4.1 −91.8 ± 10.9 0.72 ± 0.07 8
Dox-Induced (2 μg/ml) 7.4 ± 3.1 −74.7 ± 10.4 0.82 ± 0.12 8
Murine OHCs 118.06 ± 22.87 −53.81 ± 14.37 0.79 ± 0.06 21

Values are reported as means ± standard deviation of prestin-associated charge movement parameters at steady-state of prestin expression post-treatment: transiently transfected HA-prestin in HEK 293 cells and in 6 μg/ml or 2 μg/ml Dox-induced prestin-GFP in HEK 293 cells at 48hrs. Isolated murine OHCs are shown for comparison. Charge density varies with time after transfection, as well as with time after induction and inducer dose. The inducible cell line provides a tightly controlled expression of prestin function as evidenced by the small standard deviations. Vpkc and z values do not differ between the transiently transfected cell line, the inducible cell line after induction, and murine OHCs, respectively.