Table 1.
Typical protein and peptide tally following 45 min of data collection on Orbitrap Fusion and Fusion Lumos mass spectrometers for a 1μg injection of the TKO standard.
| Instrument | replicate | No. of PSMs a | Sequence coverage b | |||||
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| total | Met6 | Pfk2 | Ura2 | Met6 | Pfk2 | Ura2 | ||
| Fusion | A | 3369 | 23 | 14 | 19 | 24.1 | 12.9 | 7.3 |
| B | 3731 | 24 | 11 | 17 | 28.1 | 10.3 | 7.4 | |
| C | 3295 | 17 | 16 | 19 | 17.2 | 15.9 | 7.9 | |
|
| ||||||||
| Fusion Lumos | A | 4272 | 24 | 20 | 26 | 28.4 | 19.9 | 10 |
| B | 4316 | 26 | 21 | 23 | 33.1 | 19.3 | 8.2 | |
| C | 4272 | 22 | 20 | 25 | 24.7 | 18.4 | 9.1 | |
a
No. of PSMs refers to the number of peptide-spectral matches identified.
b
Sequence coverage is the percentage of a protein's amino acids.