Convergence of simulated binding free energies of selected complexes from 0ns to 10ns. The six complexes with PDB IDs 1Q8W, 2J75, 1VZQ, 1O2Q, 1C5,Z and 1LPZ are chosen from each of the six targets, respectively: CDK+PKA, β-glucosidase A, thrombin α, trypsin, urokinase-type plasminogen activator, and coagulation factor Xa.