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. 2016 Sep 12;6:100. doi: 10.3389/fcimb.2016.00100

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Copyright © 2016 Zhou, Feng, Zhang, Jia, Yang, Xing, Liao and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction and characterization of ΔlgtB and Δlex-1 mutants and complemented strains. (A) Parts 1–3 show the maps of the lgtB gene of wild-type SC096, the lgtB::Kan^R insertion mutant and its complemented strain. Parts 4–6 show the maps of the lex-1 gene of wild-type SC096, the lex-1::Kan^R insertion mutant and its complemented strain. (B) Part 1, Primers P1 and P4 were used to amplify upstream to downstream of lgtB from the ΔlgtB mutant strain (lane 1), the wild type SC096 (lane 2); Primers P5 and P8 were used to amplify upstream to downstream of lex-1 from the Δlex-1 mutant strain (lane 3), the wild type SC096 (lane 4). Part 2, Primers P11 and P15 were used to amplify lgtB and Gm^R from the ΔlgtB-c strain (lane 1), the wild type SC096 (lane 2); Primers P13 and P15 were used to amplify lex-1 and Gm^R from the Δlex-1-c strain (lane 3), the wild type SC096 (lane 4).