Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristic(s) | Source |
|---|---|---|
| STRAINS | ||
| E. coli DH5α | F−, ϕ80d/lacZΔM15, Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 | Laboratory collection |
| H. parasuis SC096 | Serovar 4 clinical isolate | Zhang et al., 2012b |
| ΔlgtB | SC096 ΔlgtB::KanR | This study |
| Δlex-1 | SC096 Δlex-1::KanR | This study |
| ΔlgtB-c | SC096 complemented ΔlgtB strain, GmR KanR | This study |
| Δlex-1-c | SC096 complemented Δlex-1 strain, GmR KanR | This study |
| ΔlgtB-np | SC096 ΔlgtB::GmR, in-frame non polar deletion | This study |
| ΔlgtB-oc | SC096 complemented ΔlgtB strain, GmR KanR, original locus complement | This study |
| PLASMIDS | ||
| pMD-19T (simple) | T-vector, AmpR | Takara Inc. |
| pK18mobsacB | Suicide and narrow-broad-host vector, KanR | Schäfer et al., 1994 |
| pSF115 | Kan resistance cassette-carrying complement vector, KanR | Zou et al., 2013 |
| p34S-Gm | Gm resistance cassette-carrying vector, GmR | Laboratory collection |
| pSF116 | Gm resistance cassette-carrying complement vector, GmR | This study |
| pZQ001 | A 1937bp fragment containing KanR, the upstream and downstream sequences of the lgtB gene in pMD 19T(simple), KanR | This study |
| pZQ002 | A 2076bp fragment containing KanR, the upstream and downstream sequences of the lex-1 gene in pMD 19T(simple), KanR | This study |
| pZQ003 | A 1398bp fragment containing GmR and the lgtB gene in pSF116 | This study |
| pZQ004 | A 1460bp fragment containing GmR and the lex-1 gene in pSF116 | This study |
| pZQ005 | A 1786bp fragment containing GmR, the upstream and downstream sequences of the lgtB gene in pMD 19T(simple), GmR | This study |
| pZQ006 | A 2624 bp fragment containing GmR and the lgtB gene in pMD 19T(simple), GmR | This study |