Table 2.
Sequences of the PCR primers used in this study.
| Primers | Primer sequences(5′-3′) |
|---|---|
| P1 (lgtB up-F) | ATACCGCTTGTGTGTGAGCGTCTTATATCAGCT |
| P2 (lgtB up-R) | ATGTCAATTCGGGATCCGCGTCTACTTCAGTAAGCGAA |
| P3 (lgtB down-F) | GATCGGCTTCGTCGACACGTTCGTATGTAGGAGCTGCTGGAT |
| P4 (lgtB down-R) | AGGGTAGAAGCACTCATATAG |
| P5 (lex-1 up-F) | ATACCGCTTGTGTCACCTAAGATAATATCATC |
| P6 (lex-1 up-R) | ATGTCAATTCGGGATCCGCGTATGTGAGCGTCTTATATCAG |
| P7 (lex-1 down-F) | GATCGGCTTCGTCGACACGTTCGCTCCTATTAATGGTAG |
| P8 (lex-1 down-R) | GTAGCTCAGAATGATTATCGCCA |
| P9 (Kan-F) | CGCGGATCCCGAATTGACAT TTTTATGGACAGCAAGCGAA |
| P10 (Kan-R) | ACGTGTCGACGAAGCCGATC TCAGAAGAACTCGTCAAGAA |
| P11 (lgtB comp-F) | GGTTCAAAAGAAGTTTCTATGTAAGAGTTAATTCATATTGAAGG |
| P12 (lgtB comp-R) | ATGTCAATTCGGGATCCCTATTTAAATTCAACAGTTC |
| P13 (lex-1 comp-F) | GGTTCAAAAGAAGTTTCTATGTAATATGCTATCTTAGCATAAAG |
| P14 (lex-1 comp-R) | ATGTCAATTCGGGATCC TTATTCAAAAGGAATAATAC |
| P15 (GmR-R) | GCGGTACTTGGGTCGATATC |
| P16 (GmR BamHI-F) | CGCGGATCCCGAATTGACATCGAATTGACATAAGCCTGTTC |
| P17 (GmR SalI-R) | ACGTGTCGACGAAGCCGATCTTAGGTGGCGGTACTTGGGTC |
| Expression studies by quantitative RT-PCR | |
| P18 (lgtB-F) | GACTGGTTTGAGCATTTAGATG |
| P19 (lgtB-R) | TCTAATACAGAATAGCGGG |
| P20 (rplM-F) | GTGACTGGTATGTAGTAG |
| P21 (rplM-R) | TGCCACCTACATAGCCAG |
| P22 (lgtB-F for test) | AATATCTTCTGCTTCCAAGG |
| P23 (lgtB-R for test) | CAATCAATCGGTGTTTTCTG |
| P24 (lgtB-up-F for non polar deletion) | ACCGCTTGTGTGCCGTACCATAATGTTTAG |
| P25 (lgtB-up-R for non polar deletion) | TATAATTTCCTTCAATATGAAT |
| P26 (lgtB-down-F for non polar deletion) | TGAAAAATATTACATATGTATTTG |
| P27 (lgtB-down-R for non polar deletion) | AATTGCGTTGCAGTACAAGC |
| P28 (GmR-F for non polar deletion) | ATTCATATTGAAGGAAATTATAATGTTACGCAGCAGCAACGA |
| P29 (GmR-R for non polar deletion) | CAAATACATATGTAATATTTTTCATTAGGTGGCGGTACTTGGGTC |
| P30 (lgtB-up-R for original locus complementation) | CTATTTAAATTCAACAGTTCT |
| P31 (GmR-F for original locus complementation) | AGAACTGTTGAATTTAAATAGCGAATTGACATAAGCCTGTTC |