Fig. 4. Separation and identification of antimicrobial peptides in bovine lactoferrin hydrolysates against P. syringae. Hydrolysates of bLf were confirmed using Tris tricine/SDS-PAGE (A). Reverse-phase HPLC purification of the antimicrobial peptide of bovine lactoferrin were fractionated on a column Asahipak C4P-50 (B), and antimicrobial activity of HPLC fractions was investigated using 96-well microplate method against P. syringae (C).
